The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IFNa. Next,...

The canine IFN alpha Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a canine IFN alpha ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and...

Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-alpha, IFN-beta, IFN-kappa, IFN-delta,...

Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-alpha, IFN-beta, IFN-kappa, IFN-delta,...

Specificity:, This assay has high sensitivity and excellent specificity for detection of Instant Interferon Alpha (IFNa). No significant cross-reactivity or interference between Instant Interferon Alpha (IFNa) and analogues was observed. Precision: Intra-Assay: CV<10% , Inter-Assay: CV<12% , Kit Components: 96-well...